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Wednesday, August 09, 2017
Wednesday, August 09, 2017
  • 02:00pm - 03:00pm  PB & GB Joint Division Seminar by Dr. Markita Landry PB and GB Joint Division Seminar
    Dr. Markita Landry
    Assistant Professor
    Chemical and Biomolecular Engineering
    University of California
    Berkeley, USA


    Title: Nanoparticle-Assisted Delivery of Genetic Material into Mature Plants


    Abstract:
    In light of population growth and a changing global climate, plants form the core arsenal of sustainability efforts, both at the levels of food availability and environmental remediation. Inevitably, engineering smarter plants will require creation and adoption of emerging technology that can lead to more prolific crops and more robust plants. Since the creation of the first transgenic plants, the primary challenge facing the engineering of plants is the introduction of foreign genes into plant cells through the rigid and multi-layered cell wall that is mostly composed of cellulose, hemicellulose and pectin. Current methods for mature plant transformation are labor- and timeintensive, costly, and can be suitable for a limited number of plant species, thus of limited practical applicability. We present a nanomaterial-based plant gene delivery method that can transfer biomolecules into all phenotypes of any plant species with high efficiency. We design nanomaterial formulations can penetrate the mature plant cell wall and deliver DNA gene vectors into the cytoplasm and nucleus of mature arugula plants. Nanomaterial infiltration of biological cargoes enables spatial and temporal control over genetic transformation of the plant, which can be modeled via diffusive interactions of our nanoparticle platform in plant tissues. Our results demonstrate efficient through-cell-wall DNA delivery into mature plant leaves in a passive and species-independent manner with chemically and electrostatically-modified single-walled and
    multi-walled carbon nanotube gene carriers. We also demonstrate gene silencing with a similar nanoparticle mechanism. Our platform can enable high-throughput parallel testing of plant genetic vectors in mature plants, to rapidly identify genotypes that result in phenotypes of increased plant resistance to drastic changes in climate or crop infections.  :: IRRI Events