What's happening at IRRI

Thursday Seminar

Thursday, November 17, 2016, 01:15pm - 02:15pm

 

November 17, 2016
1:15-2:15 p.m.
Havener Auditorium
IRRI

CRISPR system-mediated genome editing in rice: present status and future perspective
by:
Dr Anindya Bandyopadhyay
Scientist II-Molecular Biology
Genetics and Biotechnology

Abstract:
Genome editing using the CRISPR system has opened an interesting area of precision genome targeting in the field of gene/genome engineering research. CRISPR system with nucleases such as Cas9, Cpf1, and C2C2 provide a convenient tool for manipulating DNA in vivo through the pathway of a double strand break (DSB) and subsequent non-homologous end joining (NHEJ) or homology directed exchange. We have used codon-optimized CAS9 and Cpf1 suitable for rice, and knocked out an epidermal patterning factor gene, EPFL9. Epidermal patterning factor genes are important as they regulate stomatal biogenesis. Stomata are the pores in the plant epidermis that facilitate CO2 uptake and water loss. Thus, they play a vital role in controlling global carbon and water cycles. DNA edits generated by CRISPR/CAS9 in the epidermal patterning factor gene were stably transmitted for several generations and it was found that the Cas9 gene could be segregated out in the T3 generation. Homozygous EPFL9 mutant plants showed the expected phenotype with a drastic reduction of their leaf stomatal density when compared to wild type rice. CRISPR-Cpf1–mediated editing of the same gene, for the first time, shows that CPF1 could also be used in the plant system. This will increase the horizon of genome editing as Cpf1 could target the A/T-rich area of the genome, whereas CRISPR-Cas9 is only limited to G/C-rich locations.

Location 

Havener Auditorium, International Rice Research Institute, Los Baños, 4030 Laguna, Philippines

Map

 

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