What's happening at IRRI
Wednesday, June 15, 2016, 02:00pm - 03:00pm
Targeted mutagenesis and targeted gene insertion in rice using engineered TAL effector nucleases
Kurniawan Rudi Trijatmiko
Genetic Transformation Laboratory
Genetics and Biotechnology Division, IRRI
1400-1500H, Wednesday, 15 June 2016
Plant molecular geneticists have long dreamed of the ability to create targeted mutation and to insert the desirable gene to the target locus. Customizable sequence-specific nucleases (SSNs) can be used to create gene knockouts, which are valuable for studying gene function and for accelerating breeding for certain traits. Targeted insertion of the desirable gene to a specific genomic location suitable for stable gene expression without disrupting any endogenous gene can be facilitated by simultaneous expression of SSNs and delivery of the heterologous donor molecule. This seminar will present current progress of the work by IRRI-GTL team on the use of engineered TAL effector nucleases (TALENs) to create mutation on the eukaryotic translation initiation factor 4G (eIF4G) gene for improving rice tungro spherical virus (RTSV) resistance and to add multiple iron-biofortification genes at a predefined rice genomic site. This seminar will also describe briefly other works on QTL mapping in a population derived from a transgenic high-iron and high-zinc rice line and a high-yielding rice variety from Bangladesh, Golden Rice project and DREB project.
Rm A, DL Umali Bldg.