What's happening at IRRI

Thursday Seminar

Thursday, March 10, 2016, 01:15pm - 02:15pm

 

Thursday Seminar
March 10, 2016
1:15-2:15 p.m.
Havener Auditorium
IRRI

New fragments of old molecules

by: Andrey Grigoriev
Professor, Biology Department
Center for Computational and Integrative Biology
Rutgers University

Abstract:
I will present rather unusual suspects in the world of gene regulation: transfer RNA fragments (tRFs). Such fragments have previously escaped detection or have been generally ignored as noise due to their low number count in small RNA libraries. While the focus in the analysis of such libraries has been primarily on microRNAs (miRNAs), recent studies have reported findings of tRFs across a range of organisms. Previously, we had described age-related dynamics of miRNA loading into different RNA-induced silencing complexes (RISC) of Drosophila melanogaster using small RNA-Seq data. In this seminar, I will focus on tRFs found in the same transcriptome libraries, and their structural and functional features that make these fragments surprisingly similar to miRNAs. We found Drosophila tRF reads mapped to tRNA genes for all 20 amino acids, and detected fragments of both nuclear and mitochondrial tRNAs. Following the similarities with miRNAs, and based on complementarity with conserved Drosophila genome regions, we described potential seed sequences found in the most abundant tRFs and identified their possible targets with matches in the Drosophila melanogaster 3'UTR regions. There have been reports of finding tRFs in plant transcriptomes, suggesting the involvement of these molecules in the RNA interfering pathway across the animal and plant worlds.

Location 

Havener Auditorium, International Rice Research Institute, Los Baños, 4030 Laguna, Philippines

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