What's happening at IRRI

Special Seminar: CESD

Friday, February 12, 2016, 01:15pm - 02:15pm


CSIR-National Botanical Research Institute-
10 years of Impact through Rice Research in India

Dr. Debasis Chakrabarty
Academic Editor: PLOS One
Scientific Reports (Nature Publishing Group)
Consulting Editor: Journal of Environmental Biology,
Journal of Botanical Sciences
Genetics and Molecular Biology Div.
CSIR-National Botanical Research Institute
Rana Pratap Marg, Lucknow-226001

Abstract. 1) Rice functional genomics for drought stress: Comparative analysis of root transcriptome profiles of drought-tolerant and susceptible rice under drought stress led to identification of several putative candidate genes involved in drought response. The identification and validation of candidate genes for molecular breeding depends on acquiring data for some of the following gene features: expression pattern, chromosomal position, perceived biological function, and behavior of alleles under phenotypic selection. For this reason, we have already initiated the Rice Functional Genomics Program to develop tools and resources for functional genomics and to characterize essential genes for rice molecular breeding and genetic improvement.
2) Arsenic in rice: Biotechnological approach to reduce arsenic level in grains: Arsenic (As) contamination of the environment has emerged as a serious problem. Consequently, there is an urge to understand plants’ responses to As. Our study offers an understanding the molecular basis of arsenic toxicity and accumulation in plant parts. We have identified several candidate genes that are involved in arsenic metabolism in rice. We have generated several transgenic rice plants that accumulate less arsenic in their grains. Although measurable success, in terms of application in the field, has so far not been achieved, transgenic research has yielded promising results, which shed light on the approaches to be taken up in future endeavour. This will have tremendous societal impact related to public health consequences.
3) Plant growth promoting rhizobacteria (PGPR) mediated drought stress amelioration in rice. As an alternative to the transgenic or breeding approach, we are using stress tolerant PGPR to ameliorate the negative effects of drought stress. We have isolated a number of PGPR with ability to survive and ameliorate the abiotic stresses subjected to host plants (salt, drought and pathogen stress for rice). Recently, we have demonstrated the role of PGPR Bacillus amyloliquefaciens in rice for salt stress amelioration. Apart from it, we are also performing the microbial diversity analysis by using carbon source utilization pattern and metagenomics approach to evaluate the effects of our PGPR based bioformulations inoculation on the host rhizosphere native microflora.


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