What's happening at IRRI

Seminar: PBGB

Wednesday, March 11, 2015, 02:00pm - 03:00pm


Complementary methods for rice Zn gene discovery

Nicholas Warnock
School of Biological Sciences
Flinders University, Australia

1400-1500H, Wednesday, 11 March 2015
Umali Room A


The Stangoulis group at Flinders University is developing a range of ionomics methods for study of cereal crops. One application of these tools is the investigation of Zn loading in rice, with the aim of identifying the proteins and genes responsible for increased Zn uptake. To date, XRF, ICP-MS and Zn-specific fluorophores have been used in this work, and other technologies are now being installed. Each of these offers different levels of specificity, allowing us to quantify single or many elements simultaneously. These analyses have been paired with protein electrophoresis, protein HPLC, genome-wide analyses and soon transcriptomic analysis, which broadly represent different methods of investigating the same biochemical pathways. It is anticipated that these experiments will yield definitive results regarding control of Zn loading in rice, and that the different approaches will overlap to give increased confidence in the findings.


Rm A, Umali Bldg